ABSTRACT
<p><b>OBJECTIVE</b>To extract and purify of solamargine from Solanum nigrum, and to research its antineoplastic effects.</p><p><b>METHOD</b>S. nigrum was extracted refluently with 80% alcohol, solamargine was purified with silica gel column chromatography and recrystallization, and then conducted its structure identification and purity checks. Screened the effect on human tumor cell groth inhibition in vitro by MTT assay, and researched on the features in mice with H22 liver cancer or Ehrlich ascites tumor of solamargine.</p><p><b>RESULT</b>The concent of solamargine reached 97.9%. Solamargine had significantly inhibition on 6 tumor cells in vitro, and it had significantly inhibition on mice with H22 liver cancer or ehrlich ascites tumor in the 2.4 mg x kg(-1) dose of i.v.</p><p><b>CONCLUSION</b>Solamargine have the antineoplastic effect.</p>
Subject(s)
Animals , Female , Humans , Mice , Antineoplastic Agents, Phytogenic , Cell Line, Tumor , Mice, Inbred ICR , Solanaceous Alkaloids , PharmacologyABSTRACT
Objective To establish the method for the content determination of sinomenine in Qiwei Tongbi oral liquid. Methods Sinomenine ofQiwei Tongbi oral liquid was determined by HPLC, with the column being Waters XTerra column temperature setting at 25℃, flow rate being 1.0 ml/min, and the detective wavelength being 262 nm. Results There was a good linearity of sinomenine in the range of 0.248～2.480 μg, and the average recovery was 98.82% with RSD of 0.13%. Conclusion This method was simple, accurate, reproducible, and thus could be used for quality control of Qiwei Tongbi oral liquid.
ABSTRACT
<p><b>OBJECTIVE</b>To optimize the extraction conditions of the total amino acid from Bubali Cornu.</p><p><b>METHOD</b>An orthogonal test of L9 (3(4)) was designed to select optimum exaction conditions of the total amino acid. The influence of solvent concentration, the dosage of solvent and time of extraction were investigated with the content of total amino acid and thrombin-induced fibrin clotting time as index.</p><p><b>RESULT</b>The optimum extraction condition procedure was described as follows: the concentration of the solvent was 4 mol x L(-1), volume of solvent was 6 times amount of the materials.</p><p><b>CONCLUSION</b>The optimum exaction conditions procedure is reasonable and stable.</p>
Subject(s)
Animals , Amino Acids , Pharmacology , BuffaloesABSTRACT
Study on stability of Caitong Tablets with the constant temperature accelerated test has been carried out. Using the flavone component in Caitong Tablets as an index,their content was determined. It was predetermined that the shelf life was 4. 11 years.